p akt1 Search Results


surefire akt1 2 3 p ser473  (Revvity)
92
Revvity surefire akt1 2 3 p ser473
Surefire Akt1 2 3 P Ser473, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphorylated akt antirat antibodies  (Novus Biologicals)
93
Novus Biologicals phosphorylated akt antirat antibodies
Phosphorylated Akt Antirat Antibodies, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti p akt  (Proteintech)
96
Proteintech anti p akt
Anti P Akt, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti pakt1 2 3  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti pakt1 2 3
Anti Pakt1 2 3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p akt1 2 3 ser 473 r polyclonal antibody  (Santa Cruz Biotechnology)
97
Santa Cruz Biotechnology p akt1 2 3 ser 473 r polyclonal antibody
P Akt1 2 3 Ser 473 R Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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akt  (Proteintech)
99
Proteintech akt
A-F: Representative Western blots and quantitation are presented for phosphorylated and/or total <t>Akt,</t> PRAS40, TSC2, AMPK, <t>and</t> <t>REDD1.</t> The final panel is a representative tubulin blot demonstrating equal protein loading. Loading controls (tubulin or actin) were run for all proteins examined, but only representative data are shown. Bar graphs: were quantified for phosphorylation of each protein normalized to the total amount of the respective protein, except for REDD1 where total protein was normalized to tubulin. Control values were set to 100 arbitrary units (AU). Values are mean ± SEM. *P < 0.05 sepsis-recovery compared to pair-fed controls values; n = 17 and 10, respectively.
Akt, supplied by Proteintech, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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anti phospho akt1 2  (R&D Systems)
94
R&D Systems anti phospho akt1 2
A-F: Representative Western blots and quantitation are presented for phosphorylated and/or total <t>Akt,</t> PRAS40, TSC2, AMPK, <t>and</t> <t>REDD1.</t> The final panel is a representative tubulin blot demonstrating equal protein loading. Loading controls (tubulin or actin) were run for all proteins examined, but only representative data are shown. Bar graphs: were quantified for phosphorylation of each protein normalized to the total amount of the respective protein, except for REDD1 where total protein was normalized to tubulin. Control values were set to 100 arbitrary units (AU). Values are mean ± SEM. *P < 0.05 sepsis-recovery compared to pair-fed controls values; n = 17 and 10, respectively.
Anti Phospho Akt1 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hbax c3 egfp  (Addgene inc)
91
Addgene inc hbax c3 egfp
A-F: Representative Western blots and quantitation are presented for phosphorylated and/or total <t>Akt,</t> PRAS40, TSC2, AMPK, <t>and</t> <t>REDD1.</t> The final panel is a representative tubulin blot demonstrating equal protein loading. Loading controls (tubulin or actin) were run for all proteins examined, but only representative data are shown. Bar graphs: were quantified for phosphorylation of each protein normalized to the total amount of the respective protein, except for REDD1 where total protein was normalized to tubulin. Control values were set to 100 arbitrary units (AU). Values are mean ± SEM. *P < 0.05 sepsis-recovery compared to pair-fed controls values; n = 17 and 10, respectively.
Hbax C3 Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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akt1 pser473  (Bio-Techne corporation)
94
Bio-Techne corporation akt1 pser473
Downregulation in the FOXO1 pSer256/total FOXO1 ratio and <t>AKT1</t> <t>pSer473</t> level caused by ox-LDL treatment in HUVECs or HAECs could be partially rescued by miR-183-5p, miR-96-5p or miR-182-5p agomir treatment, which doesn’t affect the AKT1 total protein expression level (A–E). *, p < 0.05; **, p < 0.01; ***, p < 0.001.
Akt1 Pser473, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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akt ps473 nbp1 69923  (Novus Biologicals)
94
Novus Biologicals akt ps473 nbp1 69923
Downregulation in the FOXO1 pSer256/total FOXO1 ratio and <t>AKT1</t> <t>pSer473</t> level caused by ox-LDL treatment in HUVECs or HAECs could be partially rescued by miR-183-5p, miR-96-5p or miR-182-5p agomir treatment, which doesn’t affect the AKT1 total protein expression level (A–E). *, p < 0.05; **, p < 0.01; ***, p < 0.001.
Akt Ps473 Nbp1 69923, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phospho s473akt1  (Novus Biologicals)
90
Novus Biologicals anti phospho s473akt1
Downregulation in the FOXO1 pSer256/total FOXO1 ratio and <t>AKT1</t> <t>pSer473</t> level caused by ox-LDL treatment in HUVECs or HAECs could be partially rescued by miR-183-5p, miR-96-5p or miR-182-5p agomir treatment, which doesn’t affect the AKT1 total protein expression level (A–E). *, p < 0.05; **, p < 0.01; ***, p < 0.001.
Anti Phospho S473akt1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phospho akt ser473  (Proteintech)
96
Proteintech anti phospho akt ser473
Downregulation in the FOXO1 pSer256/total FOXO1 ratio and <t>AKT1</t> <t>pSer473</t> level caused by ox-LDL treatment in HUVECs or HAECs could be partially rescued by miR-183-5p, miR-96-5p or miR-182-5p agomir treatment, which doesn’t affect the AKT1 total protein expression level (A–E). *, p < 0.05; **, p < 0.01; ***, p < 0.001.
Anti Phospho Akt Ser473, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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Image Search Results


A-F: Representative Western blots and quantitation are presented for phosphorylated and/or total Akt, PRAS40, TSC2, AMPK, and REDD1. The final panel is a representative tubulin blot demonstrating equal protein loading. Loading controls (tubulin or actin) were run for all proteins examined, but only representative data are shown. Bar graphs: were quantified for phosphorylation of each protein normalized to the total amount of the respective protein, except for REDD1 where total protein was normalized to tubulin. Control values were set to 100 arbitrary units (AU). Values are mean ± SEM. *P < 0.05 sepsis-recovery compared to pair-fed controls values; n = 17 and 10, respectively.

Journal: Shock (Augusta, Ga.)

Article Title: Restorative mechanisms regulating protein balance in skeletal muscle during recovery from sepsis

doi: 10.1097/SHK.0000000000000762

Figure Lengend Snippet: A-F: Representative Western blots and quantitation are presented for phosphorylated and/or total Akt, PRAS40, TSC2, AMPK, and REDD1. The final panel is a representative tubulin blot demonstrating equal protein loading. Loading controls (tubulin or actin) were run for all proteins examined, but only representative data are shown. Bar graphs: were quantified for phosphorylation of each protein normalized to the total amount of the respective protein, except for REDD1 where total protein was normalized to tubulin. Control values were set to 100 arbitrary units (AU). Values are mean ± SEM. *P < 0.05 sepsis-recovery compared to pair-fed controls values; n = 17 and 10, respectively.

Article Snippet: Antibodies included (Cell Signaling, Beverly, MA, unless otherwise noted): S6K1, S6K1 (Thr389), rpS6, rpS6 (Ser240/244 and Ser230/235), 4E-BP1 (Bethyl Laboratories, Montgomery, TX), 4E-BP1 (Ser65), eukaryotic elongation factor (eEF)2, eEF2 (Thr56), eEF2 kinase, eEF2K (Ser366; Dr. Chris Proud), ERK (extracellular signal-regulated kinses), ERK1/2 (Thr202/Tyr204), RSK (90 kDa ribosomal S6 kinase), RSK1/2 (Ser380), REDD1 (regulated in development and DNA damage responses; ProteinTech, Chicago, IL), Akt, Akt (Thr308 and Ser473), PRAS40 (proline-rich Akt substrate of 40 kDa), PRAS40 (Thr246), eIF4B, eIF4B (Ser422), AMP-activated protein kinase-α (AMPK), AMPKα (Thr172), tuberous sclerosis complex 2 (TSC2), TSC2 (Thr1462), Unc-51 like autophagy activating kinase 1 (ULK1), ULK1 (Ser757), p62 (aka SQSTM1), light-chain 3B (LC3B)-I and –II, and MyoD and myogenin.

Techniques: Western Blot, Quantitation Assay

Downregulation in the FOXO1 pSer256/total FOXO1 ratio and AKT1 pSer473 level caused by ox-LDL treatment in HUVECs or HAECs could be partially rescued by miR-183-5p, miR-96-5p or miR-182-5p agomir treatment, which doesn’t affect the AKT1 total protein expression level (A–E). *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Journal: RSC Advances

Article Title: miR-183-96-182 clusters alleviated ox-LDL-induced vascular endothelial cell apoptosis in vitro by targeting FOXO1

doi: 10.1039/c8ra06866f

Figure Lengend Snippet: Downregulation in the FOXO1 pSer256/total FOXO1 ratio and AKT1 pSer473 level caused by ox-LDL treatment in HUVECs or HAECs could be partially rescued by miR-183-5p, miR-96-5p or miR-182-5p agomir treatment, which doesn’t affect the AKT1 total protein expression level (A–E). *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Article Snippet: For western blot analysis, primary antibodies against FOXO1 (NB100-2312), FOXO1-pSer256 (NB100-81927), AKT1 (NBP1-51602), AKT1-pSer473 (NB100-56749), and GAPDH (NB100-56875) were purchased from Bio-techne (Shanghai, China).

Techniques: Expressing

AKT1 overexpression significantly increased the AKT1 protein expression level and Ser473 phosphorylation level while reducing FOXO1 protein expression in HUVECs or HAECs with or without ox-LDL treatment (A–G). *, p < 0.05; ***, p < 0.001; ****, p < 0.0001.

Journal: RSC Advances

Article Title: miR-183-96-182 clusters alleviated ox-LDL-induced vascular endothelial cell apoptosis in vitro by targeting FOXO1

doi: 10.1039/c8ra06866f

Figure Lengend Snippet: AKT1 overexpression significantly increased the AKT1 protein expression level and Ser473 phosphorylation level while reducing FOXO1 protein expression in HUVECs or HAECs with or without ox-LDL treatment (A–G). *, p < 0.05; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: For western blot analysis, primary antibodies against FOXO1 (NB100-2312), FOXO1-pSer256 (NB100-81927), AKT1 (NBP1-51602), AKT1-pSer473 (NB100-56749), and GAPDH (NB100-56875) were purchased from Bio-techne (Shanghai, China).

Techniques: Over Expression, Expressing

AKT1 overexpression partially reduced ox-LDL-induced apoptosis in HUVECs or HAECs by upregulating miR-183-5p and miR-182-5p. (A–F) miR-183-5p and miR-182-5p were upregulated by AKT1 overexpression in HUVECs or HAECs with or without ox-LDL treatment, while miR-96-5p was not significantly influenced. (G and H) Antagomir treatment reducing the endogenous miR-183-5p or miR-182-5p expression levels partially reduced the anti-apoptotic effect of AKT1 overexpression in the HUVECs or HAECs treated by ox-LDL. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Journal: RSC Advances

Article Title: miR-183-96-182 clusters alleviated ox-LDL-induced vascular endothelial cell apoptosis in vitro by targeting FOXO1

doi: 10.1039/c8ra06866f

Figure Lengend Snippet: AKT1 overexpression partially reduced ox-LDL-induced apoptosis in HUVECs or HAECs by upregulating miR-183-5p and miR-182-5p. (A–F) miR-183-5p and miR-182-5p were upregulated by AKT1 overexpression in HUVECs or HAECs with or without ox-LDL treatment, while miR-96-5p was not significantly influenced. (G and H) Antagomir treatment reducing the endogenous miR-183-5p or miR-182-5p expression levels partially reduced the anti-apoptotic effect of AKT1 overexpression in the HUVECs or HAECs treated by ox-LDL. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: For western blot analysis, primary antibodies against FOXO1 (NB100-2312), FOXO1-pSer256 (NB100-81927), AKT1 (NBP1-51602), AKT1-pSer473 (NB100-56749), and GAPDH (NB100-56875) were purchased from Bio-techne (Shanghai, China).

Techniques: Over Expression, Expressing